Journal: Applied and Environmental Microbiology
Article Title: Protection of Bacillus pumilus Spores by Catalases
doi: 10.1128/AEM.01211-12
Figure Lengend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
Article Snippet: Catalase activity optimization was performed at various pHs and temperatures for SAFR-032 and ATCC 7061 spore suspensions (data not shown).
Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy